Supplementary Figure 1 from The E6 Oncoprotein from HPV16 Enhances the Canonical Wnt/β-Catenin Pathway in Skin Epidermis <i>In Vivo</i>

PDF file - 109K, HPV16-E6 but not HPV16-E7 oncoprotein enhances canonical Wnt/�-catenin pathway in cultured cells. (A) COS7 cells were transiently transfected with the TCF/B-catenin dependent luciferase reporter construct (TOPFLASH) and Renilla together with one or two of the following expression vectors encoding the following: HPV16-E6 (E6), HPV16-E7 (E7), Dvl2 or �-catenin (B-cat). Luciferase activity from culture cells was analyzed 48 h after transfection and is shown as normalized to total protein. HPV16-E6 but not E7 significantly induced TOPFLASH activity with or without B-cat cotransfection (A) or Dvl2 cotransfection (B) co-transfection, which suggests that E6 may positively modulate canonical Wnt signaling. The Renilla signal and empty vector were used to normalize luciferase activity.