Supplementary Figures 1-6 from Neoadjuvant Chemotherapy Induces IL34 Signaling and Promotes Chemoresistance via Tumor-Associated Macrophage Polarization in Esophageal Squamous Cell Carcinoma

S1. Measurement of the number of CD163+ cells and their expression intensities in ESCC specimens. S2. IHC staining of CSF-1, IL-34, E-cadherin, and vimentin in ESCC specimens. S3. Correlation between mRNA expression of CD163 and CSF-1 in patients with ESCC who underwent curative esophagectomy without any preoperative therapy. S4. The effect of CSF-1 expression of NAC responsivity and tumor progression after NAC in ESCC. S5. Involvement of higher fold-change of IL-34 expression after CDDP treatment to poorer chemo-sensitivity in ESCC cells. S6. Enhanced mRNA expression of IL-34 in CDDP-resistant human ESCC cells.