Effect of Probiotic-derived Extracellular Vesicles on Innate Immunity and Their Usability

Extracellular vesicles (EV) are nanoparticles secreted from cells that are involved in biological functions by transferring their cargo to target cells. Novel disease diagnostic and therapeutic methods may be developed utilizing EV derived from specific cells. In particular, mesenchymal stem cell-derived EV have several useful effects, including tissue repair. Several clinical trials are currently underway. Recent studies have demonstrated that EV secretion is not limited to mammals but also occurs in microorganisms. Since EV from microorganisms contain various bioactive molecules, elucidation of their effects on the host and their practical use is of great interest. On the other hand, for EV utilization, it is necessary to clarify their basic characteristics, such as physical properties and effects on target cells, and to develop a drug delivery system that can manipulate and utilize EV functions. However, the current state of knowledge on EV derived from microorganisms is very limited compared to that of mammalian cell-derived EV. Therefore, we focused on probiotics, microorganisms that have beneficial effects on living organisms. Since probiotics are widely used as pharmaceuticals and functional foods, the utilization of EV secreted from probiotics is expected to benefit clinical fields. In this review, we describe our research on elucidating the effects of probiotic-derived EV on the innate immune response of the host and evaluating their availability as a novel adjuvant.