Figure S9: Loss of RhoB activity increases YAP nuclear localization. from RASSF1A Suppresses the Invasion and Metastatic Potential of Human Non–Small Cell Lung Cancer Cells by Inhibiting YAP Activation through the GEF-H1/RhoB Pathway

HBEC-3 cells were transiently transfected with siNeg or siRASSF1A-1 in combination or not with siRhoB A) The invasion capacity was assessed using a BioCoat Matrigel Invasion Chamber. The relative invasion was normalized to that of the cells transfected with siNeg. B) Quantification of nuclear localization of Yap by immunofluorescence and confocal microscopy. The cells stained by Yap primary antibody (Green) followed by Alexa488 conjugated secondary antibody along with DAPI for DNA (Blue). Scale bar represents 50 µm. For all of the histograms, error bars indicate the standard error of the mean (SEM) of at least three independent experiments. *p<0.05, **p<0.01 and ***p<0.001, using an ANOVA test followed by Dunnett's test.