Supplementary Figures 1 - 15 from MicroRNA100 Inhibits Self-Renewal of Breast Cancer Stem–like Cells and Breast Tumor Development

Figure S1. Comparison of ALDH-positive population in different breast cancer cell lines and primary xenografts (UM2, MC1, UM1). Figure S2. mir-100 overexpression decreases ki67+ population in vitro. Figure S3. mir-100 overexpression inhibits the proliferation of both ALDH+ population and ALDH- population. Figure S4. mir-100 inhibits tumor growth of SUM149, MCF7 and T47D in NOD/SCID mice. Figure S5. mir-100 expression is higher in the tumor cells from mir-100 group compared to that from CTRL group. Figure S6. The percentage of ALDH+ cells in both primary CTRL and secondary CTRL tumors were increased compared to the parental cells injected. Figure S7. mir-100 expression is higher in the tumor cells from mir-100 induction groups with DOX (Late and Early) compared to that from CTRL group. Figure S8. mir-100 overexpression is necessary to maintain the inhibition of SUM159 tumor growth in NOD/SCID mice. Figure S9. mir-100 knockdown increases tumor growth in vivo. Figure S10. mir-100 inhibits metastasis of Both Total (Unseparated) and ALDH+ SUM159 cells in vivo. Figure S11. Identification of direct targets of mir-100. Figure S12. Knockdown of mir-100 targets mimic the effects of mir-100 overexpression. Figure S13. Verification of SMARCA5 and SMARCD1 level by Western blot. Figure S14. SMARCA5 and SMARCD1 are direct and functional targets of mir-100 in SUM149 cells. Figure S15. Kaplan-Meier survival curves based on the expression of SMARCA5 and SMARCD1.