Supplementary Figures 1 - 9, Tables 1 - 3 from Combination of Protein Coding and Noncoding Gene Expression as a Robust Prognostic Classifier in Stage I Lung Adenocarcinoma

PDF file - 836K, Supplemental Figure 1: Overview of the study design for the identification of candidate coding genes, development and validation of the four coding gene classifier using five independent datasets. Supplemental Figure 2: Technical validation of the microarray experiments showing Pearson�s correlation between microarray expression values and qRT-PCR expression values for each gene across all tumors that were examined.Supplemental Figure 3: Kaplan-Meier survival curves according to tertiles of the four coding gene classifier in AJCC 6th edition stage I-II lung adenocarcinoma from five independent cohorts, including the Japan (n=199, relapse-free survival), US/Norway (n=92, cancer specific mortality), Directors� (n=378, overall survival), Bhattacharjee (n=100, overall survival) and Tomida (n=92, overall survival) cohorts. P-values were estimated by log-rank test for trend. Supplemental Figure 4: For survival analysis, miR-21 expression values as measured by nCounter miRNA assays were normalized to the average of the 5 most highly expressed microRNAs in tumor tissue, excluding miR-21 itself. Supplemental Figure 5: In the Japan cohort, the four coding gene classifier and the non-coding miR-21 classifier are each significantly associated with relapse-free survival.Supplemental Figure 6: In the US/Norway cohort, the four coding gene classifier and the non-coding miR-21 classifier are each significantly associated with relapse-free survival. Supplemental Figure 7: Estimation of miR-21 copies per cell in lung adenocarcinoma tissue by PCR-based absolute quantification. Supplemental Figure 8: Total RNA content per lung tumor cell was estimated at approximately 20pg per cell based on a series of total RNA extraction from serially diluted cultured lung adenocarcinoma cell lines A549 and NCI-H23. Supplemental Figure 9: Prognostic significance of BRCA1 expression in multiple cancer types. Supplemental Table 1. Characteristics of study populations of patients from public database. Supplemental Table 2. Univariate Cox regression analysis in the Japan cohort using microarray data (AJCC TNM 6th, Stage I, n=148). Supplemental Table 3. Univariate Cox Regression analysis of the expression of six genes.