Supplementary Figure 5 from Estrogen Receptor Alpha Mediates Progestin-Induced Mammary Tumor Growth by Interacting with Progesterone Receptors at the <i>Cyclin D1/MYC</i> Promoters

PDF file - 160K, ER� increase PR activity. MDA-MB-231 cells were stably transfected with human PRB (pSG5-PRB) or with the empty vector (pSG5) and different clones were obtained. The expression of PRB was evaluated by Western blot (A), using T47D cells as a positive control of hormone receptors, or by immunofluorescence (B, bar: 30�m). (C) MDA-MB-231 pSG5 clone (3) and pSG5-PRB clone (1) cells were transiently transfected with human ER� (pSG5-HEGO) and its expression was evaluated by immnunofluorescence. Bar: 15�m. (D) MDA-MB-231 pSG5 clone (3) and pSG5-PRB clone (1) cells were co-transfected with ER� and PRE-Luc vectors. After 24 h starvation in 1% chFCS, cells were incubated with MPA for another 24 h and the activity of luciferase was evaluated.