Supplementary Figure Legend, Figures 1 - 8, Table 1 from GPR116, an Adhesion G-Protein–Coupled Receptor, Promotes Breast Cancer Metastasis via the Gαq-p63RhoGEF-Rho GTPase Pathway

PDF file - 367K, Fig S1. Screening adhesion GPCR expression in breast cancer cells. Fig S2. The GPR116 expression in knockdown or rescued breast cancer cells. Fig S3. Knockdown of GPR116 had little effect on breast cancer cell growth and apoptosis. Fig S4. The expression of GPR116 in breast cancer cell lines was quantified by Real-time PCR. Fig S5. Overexpression of GPR116 increased the activitiy of RhoA and Rac1 but not Cdc42 in 293T cells as measured by GTPase pull-down assays. Fig S6. Regulation of actin stress fibers and lamellipodia by GPR116 is RhoA and Rac1 dependent. Fig S7. The effect of RGS domain of p115RGS and Gq CT-His on GPR116 induced activity of RhoA and Rac1. Fig S8. Two additional independent experiments of Fig. 4F and Fig. 4G. Table S1. Oligonucleotide sequences of PCR primers.