Supplementary Figure 5 from miR-124 Inhibits STAT3 Signaling to Enhance T Cell–Mediated Immune Clearance of Glioma

Supplementary Figure 5 - PDF file 77K, Up regulation of miR-124 level and down regulation of p-STAT3 in peripheral blood T-cells and glioma- infiltrating T-cells after intravenous administration of miR- 124. (A) The miR-124 expression level is below the limit of detection in CD3+ T-cells isolated from the blood of non-tumor bearing C57/BL6J mice (n = 3) and GL261-bearing mice (n = 3) ( P > 0.05). Levels are shown relative to U6 snRNA. (B) miR-124 is detected in both CD3+ T-cells isolated from blood ( P < 0.05 relative to scramble control; n=3) and gliomas ( P < 0.05 relative to scramble control; n=3) 18 hours after the in vivo administration of miR-124. (C) Coinciding with the presence of miR-124, p-STAT3 expression levels were decreased in the T-cells from the peripheral blood and gliomas in the miR-124 treated mice. Representative histograms were shown. The p-STAT3 expression levels relative to isotype control in the peripheral blood T-cells in the miR-124 treated mice (1.4 � 0.2%) was down-modulated compared to scramble treated mice (17.9 � 1.9%) ( P = 0.007; n=3) and glioma-infiltrating T-cells (miR-124: 4.3 � 0.1%; scramble: 18.2 � 4.3%; P = 0.007), but not within the splenic T-cells (miR-124: 16.8 � 0.9%; scramble: 12.9 � 2.7%; P = 0.07)