Supplementary Figures 1 - 6 from Human Rhomboid Family-1 Suppresses Oxygen-Independent Degradation of Hypoxia-Inducible Factor-1α in Breast Cancer

PDF file - 440KB, Immunohistochemical analyses of RHBDF1 and HIF1a expression in breast cancer clinical specimens (S1). RHBDF1 action on HIF1a stability does not involve HIF1a mRNA levels (S2). Prediction of transmembrane topology and phosphorylation sites of RHBDF1 (S3). Effects of peptide VR56 on the expression of HIF1a targeted genes and breast cancer cell viability (S4). Inhibitory effect of RHBDF1 gene silencing in MCF7 cells on HIF1a stability (S5). Western blotting analysis showing involvement of RACK1 and RHBDF1 in HIF1a stabilization in breast cancer cell MCF7 or T47D (S6).