Supplemental Figures 1-12 from Vaccine-Elicited CD8<sup>+</sup> T Cells Cure Mesothelioma by Overcoming Tumor-Induced Immunosuppressive Environment

Supplementary Figure 1. Construction of two conventional WT1 DNA vaccines and generation of WT1-expressing AB1 malignant mesothelioma cell line. Supplementary Figure 2. Construction of HIV-1 GAG-expressing AB1 malignant mesothelioma cell line. Supplementary Figure 3. Importance of inducing p24-specific cellular responses for AB1-GAG tumor rejection. Supplementary Figure 4. Anti-tumor effect from CD8+ T cells was cross-confirmed in a B16F10 melanoma mouse model. Supplementary Figure 5. Secreted cytokines from CD8+ T cells significantly arrested AB1-GAG tumor cells at G1 phase. Supplementary Figure 6. Gating strategies for flow cytometric scatter plots. Supplementary Figure 7. Analysis of CD8+ T cell subsets in spleen and tumor. Supplementary Figure 8. Analysis of Th2 and exhaustion markers on CD8+ T cells in spleen and tumor. Supplementary Figure 9. Rapid increase of tumor-induced PD-1+CD8+ T, Tim-3+CD8+ T, MDSCs and CD4+ Treg cells. Supplementary Figure 10. Splenic and tumor-infiltrating CD8+ T cells exhibit distinct phenotypic and functional characteristics. Supplementary Figure 11. Correlation analyses of vaccine-elicited CD8+ T cells in therapeutic exp. Supplementary Figure 12. Correlation analyses in adoptive transfer experiments for inhibitory CD8+ T cells and their cytokine function. eriments.