Supplementary Figure 4 from Germline Mutations in BAP1 Impair Its Function in DNA Double-Strand Break Repair

PDF file - 35KB, Figure S4. Immunoblot analysis of PARP1/2 expression. (A) U2OS cells were transfected with control (Ctr) or PARP1 siRNA for 48 hr. Nuclear extracts were prepared and immunoblotting was performed using the indicated antibodies. (B) Knockdown of PARP2 in U2OS cells. U2OS cells were transfected with control or PARP2 siRNA for 48 h. Nuclear extracts were prepared and immunoblotted using PARP2 antibody. Actin was used as a loading control. (C) PR-DUB complex does not bind PAR in vitro. SDS-PAGE of 100 ng purified GST-BAP1, GST-ASXL1 or BAP1/ASXL1 blotted onto a nitrocellulose membrane and incubated in 250 nM 32P-labeled PAR in TBS-T. As a control, histones and BSA were included as a positive and a negative control respectively.