Supplementary Figures from Infiltrating Macrophages Promote Prostate Tumorigenesis via Modulating Androgen Receptor-Mediated CCL4–STAT3 Signaling

PDF file, 1659K, S1. Effects of THP-1 on cell migration, acinar morphogenesis, and downstream signaling in BPH-1 cells. S2. H&E staining of the anterior prostate from athymic nude mice 60 days after orthoptoic injection of either THP-1 or RWPE-1 cells. S3. Elevated pSTAT3 in mouse prostate epithelial cells (mPrE) after co-culture with mouse macrophages. S4. THP-1 cells were cultured alone for 48 hrs, and the expressions of cytokines were determined by a human cytokine array (R&D Systems). S5. qPCR (A) and Western blot analysis (B) of p53 and PTEN expression levels in RWPE-1 cells and RWPE-1/THP-1 cells after long term co-culture for 5 days. S6. The role of CCL4 and macrophage AR in the modulation of EMT markers and CCR1 expression in RWPE-1 cells. S7. Effects of macrophages AR silencing on BPH-1 cells during co-culture. S8. Generation and confirmation of MARKO/PTEN+/- mice. S9. CCL4 expression in prostate cancer does not associate with pathologic stage, lymph node metastasis, and recurrent-free survival. S10. IHC Staining of Mouse PTEN knockout prostate using an anti-Snail antibody.