Chelativorans petroleitrophicus sp. nov., a paraffin oil- degrading bacterium isolated from a mixture of oil- based drill cuttings and paddy soil

We isolated a paraffin oil- degrading bacterial strain from a mixture of oil- based drill cutting and paddy soil, and characterized the strain using a polyphasic approach. The Gram- positive, aerobic, rod- shaped and non- spore- forming strain (SCAU 2101T) grew optimally at 50 & DEG;C, pH 7.0 and 0.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that the strain represented a distinct clade in the genus Chelativorans, neighbouring Chelativorans intermedius LMG 28482T (97.1 %). The genome size and DNA G+C content of the strain were 3 969 430 bp and 63.1 mol%, respectively. Whole genome based phylogenomic analyses showed that the average nucleotide identity and digital DNA-DNA hybridization values between strain SCAU 2101T and C. intermedius LMG 28482T were 77.5 and 21.2 %, respectively. The major respiratory quinone was Q- 10. The dominant fatty acids were C19:0 cyclo & omega;8c (50.6 %), summed feature 8 (C18:1 & omega;7c and/or C18: 1 & omega;6c; 22.5 %) and C18: 0 (13.8 %). The polar lipids of the strain included phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine and diphosphatidylglycerol. Based on the results, strain SCAU 2101T was considered to represent a novel species in the genus Chelativorans, for which the name Chelativorans petroleitrophicus sp. nov. is proposed. The type strain is SCAU 2101T (= CCTCC AB 2021125T=KCTC 92067T).