Supplementary Methods, Figures 1 - 7, Tables 1 - 3 from LEO1 Is Regulated by PRL-3 and Mediates Its Oncogenic Properties in Acute Myelogenous Leukemia

Supplementary Materials and Methods. Table S1. List of upregulated and downregulated proteins identified by MS. Table S2. Primer sequences for real-time quantitative PCR. Table S3. Primer sequences used in ChIP assay on Leo1 promoter region. Figure S1. Schematic overview of SILAC approach and data analysis. Figure S2. PRL-3 upregulates Leo1 in a dose-dependent manner. Figure S3. Expression of PRL-3 and Leo1 in normal human peripheral blood sample. Figure S4. Knockdown of PRL-3 affects global histone marks. Figure S5. Leo1 promoter is not modulated by H3K27me3. Figure S6. Leo1 promoter region is regulated by JMJD2C. Figure S7. Effects of Paf1, Ctr9 or Leo1 knockdown in TF1-hPRL3 cells.