Supplementary Figure 1 from Expression of AR-V7 and ARv<sup>567es</sup> in Circulating Tumor Cells Correlates with Outcomes to Taxane Therapy in Men with Metastatic Prostate Cancer Treated in TAXYNERGY

Assay sensitivity in 22RV1 prostate cancer cells spiked-in healthy donor blood run through the GEDI device. AR-FL (blue) and AR-V7 (red) mRNA expression (#copies per sample) was determined by ddPCR in varying amounts of 22RV1 cells in the presence of healthy donor blood run through the GEDI device. The assay, reliably and reproducibly detects both transcripts in single spiked-in cells. The table below the graph shows the raw data (copy number) for each transcript per condition. Healthy donor blood PBMCs alone were used as a control. AR, androgen receptor; ddPCR, droplet digital polymerase chain reaction; FL, full length; GEDI, geometrically enhanced differential immunocapture; PBMC, peripheral blood mononuclear cell.