Supplementary Figure 4 from Distinct Effects of Ligand-Induced PDGFRα and PDGFRβ Signaling in the Human Rhabdomyosarcoma Tumor Cell and Stroma Cell Compartments

PDF file - 80K, Myogenic differentiation is impaired in RD cells devoid of PDGF activity. RD cells were cultured for 72 hours under conditions facilitating myogenic differentiation. A, quantification of SA-βgal-positive senescent cells in the presence of CP-673,451 or vehicle. B, cell elongation as a morphological sign of myogenic differentiation in the presence of CP-673,451 or vehicle. C, qRT-PCR analysis for the two myogenic transcripts MYL1 and MYOGENIN and the myogenic repressor HEY-1, a mediator of Notch signaling, in the presence of CP-673,451 or vehicle (0.1% DMSO) (left panel). The right panel shows expression of the same myogenic transcripts in non-differentiated cells and in cells where differentiatiation was induced in the absence of vehicle. All expression values were normalized to the expression in non-differentiated cells (right panel, left bar). Data are presented � SD (*P<0.05, ***P<0.001, Student's t test).