Supplementary Methods from The Alkylating Chemotherapeutic Temozolomide Induces Metabolic Stress in <i>IDH1</i>-Mutant Cancers and Potentiates NAD<sup>+</sup> Depletion–Mediated Cytotoxicity

Kensuke Tateishi,Fumi Higuchi,Julie J. Miller, Mara V.A. Koerner,Nina Lelic,Ganesh M. Shankar,Shota Tanaka,David E. Fisher,Tracy T. Batchelor,A. John Iafrate,Hiroaki Wakimoto,Andrew S. Chi,Daniel P. Cahill

crossref（2023）

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摘要

Detailed information regarding Creation of Glioma Tumorsphere Lines, Cell culture and Reagents, IDH1-R132H Cell Line Generation, shRNA and control shRNA Cell line Generation, Western Blot Analyses, Cell Viability, Cytotoxicity Analysis and Clonogenicity assay, PARP activity assessment, NAD+ quantitation, Animal Studies and Immunohistochemistry

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Supplementary Methods from The Alkylating Chemotherapeutic Temozolomide Induces Metabolic Stress in &lt;i&gt;IDH1&lt;/i&gt;-Mutant Cancers and Potentiates NAD&lt;sup&gt;+&lt;/sup&gt; Depletion–Mediated Cytotoxicity

Supplementary Methods from The Alkylating Chemotherapeutic Temozolomide Induces Metabolic Stress in <i>IDH1</i>-Mutant Cancers and Potentiates NAD<sup>+</sup> Depletion–Mediated Cytotoxicity