Supplementary Method, Supplementary Tables, and Supplementary Figures from Photodynamic Therapy Synergizes with Irinotecan to Overcome Compensatory Mechanisms and Improve Treatment Outcomes in Pancreatic Cancer

(i) Supplementary Methods 1. Synthesis of L-BPD and L-IRI via freeze-thaw extrusion method 2. Pharmacokinetics and biodistribution of L-BPD and L-IRI 3. In-vitro quantification of changes in ABCG2 expression using immunofluorescence 4. Ultrasound Imaging 5. Histology and immunofluorescence of tumor samples 6. Quantification of H&E and immunofluorescence biomarkers 7. Determination of Synergism (ii) Supplementary Tables Supplementary Table S1. Comparison of PDT and irinotecan doses used in this study versus in clinic Supplementary Table S2. List of antibodies used for immunofluorescence Supplementary Table S3. The synergistic effect of combination PDT and L-IRI Supplementary Table S4. Physical parameters of L-BPD and L-IRI (iii) Supplementary Figures and Legends Supplementary Fig. S1. Treatment schedule and longitudinal monitoring of PanCa tumors in vivo Supplementary Fig. S2. Sub-cytotoxic PDT leads to decreased levels of ABCG2 and increased intracellular irinotecan in AsPC-1 cells Supplementary Fig. S3. Tumor growth kinetics of MIA PACa-2 and AsPC-1 in vivo Supplementary Fig. S4. Change in mouse body weight after combination treatment