Supplemental Figure S4 from Treatment-Induced Tumor Cell Apoptosis and Secondary Necrosis Drive Tumor Progression in the Residual Tumor Microenvironment through MerTK and IDO1

Supplemental Figure S4. THP-1 cells differentiated with M-CSF were assessed for their ability to polarize to an M1 phenotype in response to LPS treatment. Cells were treated for 24 hrs. with LPS (700ng/mL). Activation was analyzed via flow cytometry comparing CD86 expression on LPS treated cells to untreated cells and quantified. Differentiated THP-1 cells were treated with either BMS777607 (final concentration of 1uM) or DMSO (1:1000) for 2hrs. then co-cultured with BT474 cells [treated with either DMSO (1:1000) or Lapatinib (1uM) for 2hrs.] for a total of 4 hours, before being collected for RNA isolation and qPCR. TUNEL analysis of tumors harvested from MMTV-Neu tumor-bearing mice at treatment day 2 and treatment day 7. Representative images are shown.