Supplemental info from Vitamin C Sensitizes Melanoma to BET Inhibitors

This file contains the following supplementary figures, tables and other materials. Figure S1: The expression of BRD2, BRD3 and BRD4 in melanocytes and melanoma cells. Figure S2: Knocking down the expression of BRD4 by siRNA. Figure S3: The effect of ascorbate on the expression of BRDs. Figure S4: hMeDIP-qPCR of HAT1 in its promoter and gene body. Figure S5: Decreased acetylation of soluble H4 by ascorbate treatment. Figure S6: The effect of ascorbate on H4K8ac and H4K16ac. Figure S7: Isobologram analysis of JQ1 and ascorbate interaction in A2058 cells. Figure S8: Ascorbate sensitizes murine melanoma cell B16-F10 to BETi. Table S1. Primers for hMeDIP-qPCR of HAT1. Table S2: Epigenetic drug screening panel. Table S3: Ascorbate decreases the EC50 of BET inhibitors in melanoma cell lines. Supplementary Material and Methods Supplementary reference