Shoot differentiation from Dendrocalamus brandisii callus and the related physiological roles of sugar and hormones during shoot differentiation.

Only a few calli regeneration systems of bamboos were successfully established, which limited the research on physiological mechanism of callus differentiation. In this study, we successfully established the callus differentiation systems of Dendrocalamus brandisii via seeds. The results showed that the best medium for callus induction of D. brandisii seeds was basal MS media amended with 5.0 mg L-1 2,4-D and 0.5 mg L-1 KT, and the optimal medium for shoot differentiation was the basal MS media supplemented with 4.0 mg L-1 BA and 0.5 mg L-1 NAA. Callus tissues had apparent polarity in cell arrangement, and developed their own meristematic cell layers. α-amylase, STP and SUSY played a dominant role in carbohydrates degradation in callus during shoot differentiation. PPP and TCA pathways up-regulated in the shoot-differentiated calli. The dynamics of BA and KT contents in calli was consistent with their concentrations applied in medium. IAA synthesis and the related signal transduction were down-regulated, while the endogenous CTKs contents were up-regulated by the exogenous CTKs application in shoot-differentiated calli, and their related synthesis, transport and signal transduction pathways were also up-regulated. The downregulated signal transduction pathways of IAA and ABA revealed that they did not play the key role in shoot differentiation of bamboos. GAs also played a role in shoot differentiation based on the down-regulation of DELLA and the up-regulation of PIF4 genes. The overexpression of DbSNRK2 and DbFIF4 genes further confirmed the negative role of ABA and the positive role of GAs in shoot differentiation.