How CD4 + T Cells Transcriptional Profile Is Affected by Culture Conditions: Towards the Design of Optimal In Vitro HIV Reactivation Assays.

Giuseppe Rubens Pascucci,Elena Morrocchi,Chiara Pighi,Arianna Rotili, Alessia Neri,Chiara Medri,Giulio Olivieri,Marco Sanna,Gianmarco Rasi,Deborah Persaud,Ann Chahroudi,Mathias Lichterfeld,Eleni Nastouli,Caterina Cancrini,Donato Amodio,Paolo Rossi,Nicola Cotugno,Paolo Palma

Biomedicines（2023）

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摘要

These results suggest that for studies aiming at quantifying the magnitude of biological mechanisms under T cell activation, the autologous plasma could better approximate the in vivo environment. Conversely, if the study aims at defining qualitative aspects, then RPMI culture could provide more evident results.

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关键词

CD4+ T cells,HIV reactivation,Oxford Nanopore technologies,PMA/ionomycin stimulation,RNA sequencing,RPMI,T cell activation,TCR signaling cascade,autologous plasma,in vitro cultures

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