A Metagenomic Library Cloning Strategy That Promotes High-Level Expression of Captured Genes to Enable Efficient Functional Screening.

Michelle H. Rich,Abigail V. Sharrock,Timothy S. Mulligan,Jeff S. Mumm,David F. Ackerley,Frazer Matthews,Alistair S. Brown, Hannah R. Lee-Harwood,Elsie M. Williams,Janine N. Copp,Rory F. Little, Jenni J. B. Francis,Claire N. Horvat,Luke J. Stevenson,Jeremy G. Owen,Meera T. Saxena, David F. Auckerly

Cell chemical biology（2023）

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关键词

bacterial start codons,environmental DNA screening,FatI restriction cloning,functional metagenome screening,metronidazole,niclosamide,nitroreductase,synthetic biology,targeted cell ablation

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