Towards standardizing basophil identification by flow cytometry.

Soren Ulrik Sonder,Matthew Plassmeyer,Denise Loizou,Oral Alpan

Frontiers in allergy（2023）

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摘要

In flow cytometry assays, two surface markers should be used for identifying basophils and if a very pure basophil fraction is desired a third marker can be considered. In our hands the approaches that included CD123 in combination with either CD193, HLADR or FcɛRI performed the best.

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关键词

CBC (complete blood count),basophil actication test,basophil activating test (BAT),basophil identification markers,flow cytometry

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