Sperm Cryopreservation of the African Turquoise Killifish Nothobranchius furzeri.
Cold Spring Harbor protocols(2023)
摘要
Sperm cryopreservation is an essential method for the genetic preservation and long-term storage of wild-type and transgenic animal stocks. In addition, it allows for the synchronization of gamete availability and the transport and sharing of lines between different laboratories. Here, we describe a protocol developed in our laboratory for the extraction and cryopreservation of killifish (Nothobranchius furzeri) sperm.
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