Targeting Caspase-3 Gene in rCHO Cell Line by CRISPR/Cas9 Editing Tool and Its Effect on Protein Production in Manipulated Cell Line.

Amirabbas Rahimi,Morteza Karimipoor,Reza Mahdian,Atefeh Alipour,Sadi Hosseini,Hooman Kaghazian,Abdolrahim Abbasi,Hosein Shahsavarani,Mohammad Ali Shokrgozar

Iranian journal of pharmaceutical research : IJPR（2022）

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摘要

We understood that CRISPR/Cas9 could be effectively applied to suppress the expression of the caspase-3 gene and rescue CHO cells from apoptosis induced by cell stress and metabolites. The CRISPR/Cas9 system-assisted caspase-3 gene ablation can potentially increase erythropoietin yield in CHO cells.

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关键词

Apoptosis,CHO cells,CRISPR/Cas9,Caspase-3,Gene Editing

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