CODEX ® Multiplexed Imaging – tissue staining in TMA and whole tissue FFPE sections v1

CODEX technology allows for highly multiplexed analysis of + 40 proteins on the same section relying on DNA-conjugated antibodies being commercialized by Akoya Biosciences (former name CODEX®, current name PhenoCycler™). This detailed protocol describes an adapted protocol, from CODEX User Manual Rev C , for tissue staining in Tissue MicroArrays (TMA) and whole tissue Formalin Fixed Paraffin Embedded (FFPE) sections to run into the CODEX system which is used in Emma Lundberg research group at Science for Life Laboratory; KTH - Royal Institute of Technology.