Indirect immunofluorescence - tissue staining in TMA and whole tissue FFPE sections v1

Immunofluorescence staining allows detection and localization of antigens in different tissue types providing high sensitivity. The indirect immunofluorescence protocol is based on the principle of using a primary antibody binding to the target epitope, and a fluorophore-tagged secondary antibody that recognizes and binds to the primary antibody. This methods provides signal amplification allowing detection of several targets in the same tissue sample. This detailed protocol describes an adapted protocol, from An atlas of the protein-coding genes in the human, pig, and mouse brain article, for tissue staining in Tissue MicroArrays (TMA) and whole tissue Formalin Fixed Paraffin Embedded (FFPE) sections which is used in Emma Lundberg research group at Science for Life Laboratory; KTH - Royal Institute of Technology.