Seroprevalence and Factors Associated with Toxoplasma gondii , Neospora caninum , and Besnoitia besnoiti Infections in Cattle and Goats in Selangor, Malaysia.

Apicomplexan parasites such as , , and are widely recognized as causes of production diseases in ruminants. This study aimed to investigate the serological occurrence of , , and in cattle and goats from smallholder farms in Selangor, Malaysia. A cross-sectional study was conducted on 19 farms by collecting 404 bovine (n = 225) and caprine (n = 179) serum samples, which were then essayed for , , and antibodies using commercially available ELISA test kits. Farm data and animal characteristics were documented, and the data were analyzed using descriptive statistics and logistic regression models. The seroprevalence of at animal and farm levels in cattle was 5.3% (95% CI 1.2-7.4%) and 36.8% (95% CI 22.4-58.0%), respectively. Animal-level seropositivity for was 2.7% (95% CI 0.4-4.2%) and 5.7% for (95% CI 1.3-9.4%) with corresponding farm-level seropositivity of 21.0% and 31.5%, respectively. For the goat samples, a high animal- (69.8%; 95% CI 34.1-82.0%) and farm-level (92.3%) seropositivity was recorded for , but was relatively lower for antibodies, at 3.9% (95% CI 1.5-6.2%) and 38.4% (5/13). The factors associated with seropositivity were older animals (above 12 months) (OR = 5.3; 95% CI 1.7-16.6), semi-intensive farms (OR = 2.2; 95% CI 1.3-6.2), the presence of either dogs or cats (OR = 3.6; 95% CI 1.1-12.3), a large herd size (>100 animals) (OR = 3.7; 95% CI 1.4-10.0), and a single source of replacement animals (OR = 3.9; 95% CI 1.6-9.6). These findings are vital in developing effective control measures against these parasites in ruminant farms in Selangor, Malaysia. More national epidemiological research is required to elucidate the spatial distribution of these infections and their potential impact on Malaysia's livestock industry.