Performance evaluation of the bio-rad BioPlex 2200 multiplex system in the detection of measles, mumps, rubella, and varicella-zoster antibodies

•Measles, mumps, rubella, and varicella-zoster (MMRV) immunity testing is important in occupational screening of healthcare workers and at-risk populations.•Multiplex fluorescent immunoassay (MFI) benefits from high throughput testing of multiple antibodies simultaneously.•Bio-Rad BioPlex 2200 MFI demonstrated good agreement with the Bio-Rad Evolis Twin Plus measles, mumps, and varicella-zoster (MMV) IgG assay and the Abbott Architect Rubella IgG assay.•This is the first report that includes imprecision analysis of the Bio-Rad BioPlex MMRV IgG assay against two EIA methods.