A surface-enhanced Raman scattering (SERS) biosensor fabricated using the electrodeposition method for ultrasensitive detection of amino acid histidine

•SERS-active substrates were prepared using the electrodeposition method, which is simple and repeatable.•By depositing silver nanoparticles on FTO glass substrates by electrodeposition method, the fabricated biosensor showed high sensitivity, stability, and reproducibility.•Rapid, accurate, ultrasensitive, and low-cost detection of the amino acid histidine up to a concentration of 10−9 resulted using the developed SERS biosensor prepared by coating silver nanoparticles on FTO glass substrates and electrodeposition method.•FDTD simulations were carried out, and the electric field distributions as well as the enhancement factor of the developed SERS-active substrates were obtained.