Investigation and Screening of Mixed Microalgae Species for Lipase Production and Recovery using Liquid Biphasic Flotation Approach

Journal of the Taiwan Institute of Chemical Engineers(2023)

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摘要
•Microalgae desmodesmus and scenedesmus sp. were investigated as sources of lipases.•Lipase enzyme was recovered from wet microalgae using alcohol/salt LBF.•P-nitrophenol-acyl esters was used to screen lipases for desirable activities.•Optimal system produces recovery yield of 70.3% with purification factor of 7.5.•Lipases successfully recovered by using recycling liquid biphasic flotation.
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关键词
Microalgae lipase,Purification,Liquid biphasic flotation,Pharmaceutical,P-nitrophenyl
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