Inducible positive amplification regulation coupled with the double-strand specific nuclease for FzD5 mRNA assay

Gaihua Cao,Keyi Long,Yue Qiu,Yi Ma,Hui Qin,Danqun Huo,Mei Yang,Caihong Shen,Changjun Hou

Microchemical Journal（2023）

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摘要

•IPAR mode formed an amplification network by RCA and SDA to improve sensitivity.•IPAR mode expanded the application of RCA and SDA and improved their efficiency.•The involvement of DSN enhanced the specificity of the biosensor.•The biosensor provided novel and sensitive methods for mRNA detection.

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关键词

Inducible positive amplification regulation,Double-strand Specific Nuclease (DSN),Rolling circle amplification (RCA),Strand displacement amplification (SDA),Frizzled receptor 5 (FzD5) mRNA

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