A sensitive dual-signal electrochemiluminescence immunosensor based on Ru(bpy) 3 2+ @HKUST-1 and Ce 2 Sn 2 O 7 for detecting the heart failure biomarker NT-proBNP.

A sensitive dual-signal electrochemiluminescence (ECL) immunosensor was proposed based on Ru(bpy)@HKUST-1/TPA and CeSnO/KSO probes for detecting the NT-proBNP biomarker of heart failure. HKUST-1 with a high specific surface area facilitates the loading of more Ru(bpy), effectively improving the anodic signal intensity, while the emerging CeSnO emitter displays a potential-matching cathodic emission with moderate intensity. Two ECL probes were characterized with field emission scanning electron microscopy, X-ray diffraction, XPS, FT-IR spectroscopy and UV-Vis diffuse reflectance spectroscopy. This dual-signal immunosensor has a wide linear range (5 × 10-1 × 10 ng mL) and a low quantitative detection limit, simultaneously showing high sensitivity, stability and reproducibility, as well as the detection capability of actual serum samples. This dual signal-calibrated immunoassay platform not only reduces the false positive rate of detection results but also provides a promising method for the early diagnosis of heart failure.