Trehalose-6-phosphate synthase regulates chitin synthesis in Mythimna separata .

Trehalose is a substrate for the chitin synthesis pathway in insects. Thus, it directly affects chitin synthesis and metabolism. Trehalose-6-phosphate synthase (TPS) is a crucial enzyme in the trehalose synthesis pathway in insects, but its functions in remain unclear. In this study, a -encoding sequence in () was cloned and characterized. Its expression patterns at different developmental stages and in diverse tissues were investigated. The results indicated that was expressed at all analyzed developmental stages, with peak expression levels in the pupal stage. Moreover, was expressed in the foregut, midgut, hindgut, fat body, salivary gland, Malpighian tubules, and integument, with the highest expression levels in the fat body. The inhibition of expression RNA interference (RNAi) resulted in significant decreases in the trehalose content and TPS activity. It also resulted in significant changes in ( and ) expression, and significantly decrease the chitin content in the midgut and integument of . Additionally, the silencing of was associated with a significant decrease in weight, larval feed intake, and ability to utilize food. It also induced abnormal phenotypic changes and increased the mortality and malformation rates. Hence, is important for chitin synthesis. The results of this study also suggest RNAi technology may be useful for enhancing the methods used to control infestations.