Analysis of Industrial Bacillus Species as Potential Probiotics for Dietary Supplements (vol 11, 488, 2023)

So far, species bacteria are being used as bacteria concentrates, supplementing cleaning preparations in order to reduce odor and expel pathogenic bacteria. Here, we discuss the potential of species as 'natural' probiotics and evaluate their microbiological characteristics. An industrial microbiological concentrate CS-4 of mixed species cultures was tested, which may be a promising bacteria source for food probiotic preparation for supplementary diet. In this study, antagonistic activities and probiotic potential of species, derived from an industrial microbiological concentrate, were demonstrated. The cell free supernatants (CFS) from mostly inhibited the growth of foodborne pathogenic bacteria, such as O157:H7 ATCC 35150, KCCM 12021, and KCCM 11335, while some of strains showed synergistic effect with foodborne pathogenic bacteria. Moreover, strains identified by the MALDI TOF-MS method were found sensitive to chloramphenicol, kanamycin, and rifampicin. and displayed the least sensitivity to the other tested antibiotics, such as ampicillin, ampicillin and sulfbactam, streptomycin, and oxacillin and bacitracin. Furthermore, some of the bacterial species detected extended their growth range from the mesophilic to moderately thermophilic range, up to 54 °C. Thus, their potential sensitivity to thermophilic TP-84 bacteriophage, infecting thermophilic , was tested for the purpose of isolation a new bacterial host for engineered bionanoparticles construction. We reason that the natural environmental microflora of non-pathogenic species, especially , can become a present probiotic remedy for many contemporary issues related to gastrointestinal tract health, especially for individuals under metabolic strain or for the increasingly growing group of lactose-intolerant people.