Hypoxia fluorescence imaging probe and preparation method and application thereof

The invention provides a hypoxic fluorescence imaging probe as well as a preparation method and application thereof, wherein the hypoxic fluorescence imaging probe has a structure as shown in a formula I, and is named as 1-(1-(2-nitroimidazole)-3-[(4-aminomethyl)-azacyclohexane]-2-propanol)-6-(4-nitrobenzo-2-oxa-1,3-diazole)-lysine, PLN for short; the probe disclosed by the invention can realize specific fluorescence imaging of hypoxic cells by virtue of different macromolecular combinations in different hypoxic environments by virtue of nitroimidazole groups; furthermore, the primary amino groups in the molecules are beneficial to further modification of the molecules.