Tandem Repeat Generation and Novel Isothermal Amplification Based on Nonspecific Tailing and Replication Slippage.

Guangcheng Luo,Hongfei He,Dongsheng Wang, Shanshan Liu,Shisu Tian, Miaomiao Chen,Qiang Wang, Changsong Zhao,Zhengwei Leng,Lingmi Hou,Xiaolan Guo

Clinical chemistry（2023）

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摘要

We discovered the mechanism for Bst-mediated nonspecific TRs generation and developed a novel isothermal amplification assay (BASIS), which can detect nucleic acids with high sensitivity and specificity.

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关键词

isothermal amplification,nonspecific amplification,nonspecific tailing,replication slippage,tandem repeats

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