Evaluation of relative efficiency of PDT photosensitizers in producing hydroxyl radicals and singlet oxygen in aqueous media using a UV-visible spectroscopy pNDA dosage.

In order to improve the performance of PDT, it is important to develop new photosensitizers that induce the formation of both hydroxyl radicals and singlet oxygen. In this work, we developed and validated the experimental conditions and reproducibility for the evaluation of relative efficiency of hydroxyl radicals and singlet oxygen production by studying the bleaching of p-nitrosoaniline (pNDA) using a continuous flow UV-visible spectroscopy method in presence of photosensitizers in PBS media. Rapid data sampling made possible to analyze the kinetics of the bleaching by using a mathematical modeling. The pNDA dosage is specific of hydroxyl radicals' production without l-histidine and of singlet oxygen production in presence of l-histidine. A statistical approach is used to precisely evaluate the reliability of the results and to be able to compare different photosensitizers between them such as Methylene Blue and Brillant Blue G.