Examining binding capacity of aptamers based on fluorescence image segmentation

In order to meet the needs of verifying binding capacities in aptamer selection experiments, a binding capacity examination device for aptamers and targets based on the fluorescence image segmentation was developed IP 8.46.247.10 On: Wed, 14 Dec 2022 07:26:26 Copyright: American Scientific Publishers in this paper. The fluorescence imaging module is used to collect images of aptamers binding to target cells, Del vered by Ingenta and then the average fluorescence intensity of a single cell is calculated using methods of the cell image segmentation and the fluorescence image registration. By comparing the fluorescence intensity, the binding capacity of aptamers can be investigated and the progress of selection experiments can be monitored. The application value of this paper is to provide a new method for the dynamic monitoring of the aptamer binding performance during the automatic selection.