Double Digest Restriction-Site Associated DNA Sequencing (ddRADseq) Technology.

Natalia Cristina Aguirre,Carla Valeria Filippi,Pablo Alfredo Vera,Andrea Fabiana Puebla,Giusi Zaina,Verónica Viviana Lia,Susana Noemí Marcucci Poltri,Norma Beatriz Paniego

Methods in molecular biology (Clifton, N.J.)（2023）

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摘要

Double digest restriction-site associated DNA sequencing (ddRADseq) technology combines genome reduced representation by digestion with two restriction enzymes and next generation sequencing (NGS) to obtain thousands of markers (SNP, SSR, and InDels) and genotype tens to hundreds of samples simultaneously. In this chapter, we describe a 96-plex derived ddRADseq protocol that can be set up to obtain different depth of coverage per locus and can be exploited to model and non-model plant species.

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关键词

Genotyping by sequencing,InDels,Molecular markers,Next generation sequencing,RADseq,Reduced representation libraries,SNP,SSR

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