Defective porphyrin-based metal-organic framework nanosheets derived from V2CTx MXene as a robust bioplatform for impedimetric aptasensing 17?-estradiol

An electrochemical aptasensor was prepared for the efficient, sensitive, and selective detection of 17 beta-estradiol. The sensor was based on a defective two-dimensional porphyrin-based metal-organic framework derived from V2CTx MXene. The resulting metal-organic framework nanosheets benefited from the advantages of V2CTx MXene nanosheets and porphyrin-based metal-organic framework, two-dimensional porphyrin-based metal--organic framework nanosheets demonstrated amplified electrochemical response and enhanced aptamer-immobilization ability compared with V2CTx MXene nanosheets. The sensor's detection limit was ultralow at 0.81 fg mL-1 (2.97 fM), and the 17 beta-estradiol concentration range was wide, thereby outperforming most re-ported aptasensors. The high selectivity, superior stability and reproducibility, and excellent regeneration per-formance of the constructed aptasensor indicated its remarkable potential application for 17 beta-estradiol determination in diverse real samples. This aptasensing strategy can be used to analyze other targets by replacing the corresponding aptamer.