Ethylicin Inhibition of Xanthomonas oryzae pv. oryzicola In Vitro and In Vivo .

Infestation of rice with the bacterium pv. oryzicola () causes the serious disease bacterial leaf streak (BLS). We studied the effect of ethylicin, a broad-spectrum bactericide, on both and . Ethylicin increases the defensive enzyme activities and defensive genes expression of rice. Ethylicin also significantly inhibited activity compared with other commercial bactericides. The half-maximal effective concentration (EC) of ethylicin was 2.12 μg/mL. It has been shown that ethylicin can inhibit quorum sensing through the production of extracellular polysaccharides and enzymes, which disrupt the cell membrane. We used proteomic analysis to identify two oxidative phosphorylation pathway proteins (ACU12_RS13405 and ACU12_RS13355) which affected the virulence of and validated them using quantitative real-time polymerase chain reaction (qRT-PCR). The results indicate that ethylicin can increase the defense responses of rice and control proliferation.