A comprehensive in vitro exploration into the interaction mechanism of coumarin derivatives with bovine hemoglobin: Spectroscopic and computational methods

•Binding of coumarin derivatives with bovine hemoglobin have been investigated using biophysical techniques.•Static quenching mechanism is involved in the binding interactions of coumarins with bovine hemoglobin.•Binding constant was found moderate in nature.•Secondary structure of the protein was perturbed during ligand binding.•Results from computational studies correlated well with the experimental findings.