Mechanism study of Cordyceps sinensis alleviates renal ischemia-reperfusion injury

Cordyceps sinensis (C. sinensis) is a kind of traditional Chinese medicine commonly used to protect renal function and relieve kidney injury. This study aimed to reveal the renal protective mechanism of C. sinensis in renal ischemia-reperfusion injury (RIRI). First, we obtained 8 active components and 99 common targets of C. sinensis against RIRI from public databases. Second, we have retrieved 38 core targets through STRING database analysis. Third, Gene Ontology analysis of 38 core targets is indicated that C. sinensis treatment RIRI may related hormone regulation, oxidative stress, cell proliferation, and immune regulation. Kyoto Encyclopedia of Genes and Genomes enrichment analysis of 38 core targets is indicated that C. sinensis treatment RIRI may involve in PI3K-Akt, HIF-1, and MAPK signaling pathways, as well as advanced glycation end product (AGE)-receptor for AGE (RAGE) signaling pathway in diabetic complications. Lastly, molecular docking was used to detect the binding activity and properties of active components and core target using molecular docking. And the results showed that eight active components of C. sinensis had low affinity with core targets. In conclusion, C. sinensis may improve RIRI by regulating oxidative stress and immunity through PI3K-Akt, HIF-1, and MAPK pathways.