Preliminary Report on CSN3 and LGB genes Polymorphism among Two Romanian Cattle Breeds

The aim of the current study was to evaluate the genetic polymorphism of A and B variants at the CSN3 (κ-casein) and LGB (β-lactoglobulin) loci in Romanian Spotted and Romanian Brown breeds using PCR-RFLP and PCR-HRM genotyping methods. Altogether, 280 cattle from Arad county were genotyped. The most frequent allele at the CSN3 locus was A in Romanian Spotted breed (0.723) while the B allele was the most common in the Romanian Brown (0.694). For LGB locus the A and B alleles recorded similar frequencies for Romanian Spotted (0.5141 and 0.4859) compared to Romanian Brown breed where the B allele was the most prevalent (0.5970). Knowledges regarding genetic information of breeds related to milk protein are valuable for economic reassertion of cattle breeds, particularly autochthonous ones. Further, the results would be useful in future breeding programs for the studied population, aimed to improve cattle genetic structure for milk quality traits.