The validation of Goldeneye™ DNA ID 22NC kit and the genetic polymorphism of 21 short tandem repeat loci in the Chinese Hunan Han population

Goldeneye™ DNA ID 22NC Kit is a novel short tandem repeat (STR) genotyping system that investigate 20 non-CODIS loci (D4S2366, D6S477, D22GATA198B05, D15S659, D8S1132, D3S3045, D14S608, D17S1290, D3S1744, D2S441, D18S535, D13S325, D7S1517, D10S1435, D11S2368, D19S253, D1S1656, D7S3048, D10S148, and D5S2500), a CODIS locus (D3S1358), and a sex-determining locus amelogenin in one assay. In the present study, this STR genotyping system was validated according to the guidelines of “Validation Guidelines for DNA Analysis Methods (2016)” updated by the Scientific Working Group on DNA Analysis Methods. A series of tests, such as polymerase chain reaction-based studies, sensitivity, inhibitors, DNA mixture, species specificity, precision and accuracy evaluation, stutter percentage, and peak height ratio, was conducted. The genetic polymorphism of 21 STR loci that included in the 22NC system was also investigated in the Chinese Hunan Han population. The validation results demonstrated that Goldeneye™ DNA ID 22NC Kit is a robust and reliable identification assay as required for genotyping in kinship analysis and forensic investigation. The 21 STR loci in this kit also showed a high level of genetic polymorphism for the Hunan Han population. Therefore, it can be used for forensic applications and population studies.