Enhancing the co-production of sugars from sugarcane bagasse via CuCl2-catalyzed organosolv pretreatment and additives

In this work, the effects of CuCl2-catalyzed organsolv on sugarcane bagasse pretreatment and enzymatic hydrolysis were investigated under different reaction conditions. The majority of hemicellulose and lignin were degraded when 0.025 mol/L CuCl2 were used in the pretreatment at 160 °C for 10 min, resulting in 89.25% of glucose yield after hydrolysis for 72 h, which was revealed by structure alteration, which was confirmed by different characterization analysis. Furthermore, the effect of additives (Tween 80, BSA, tea saponin, and xylanse) on enzymatic hydrolysis was determined. Except for xylanse, the glucose and xylose yields were enormously enhanced by adding additives, and their increased yield rose initially and then declined with the extending of hydrolysis time. Combined with the pretreatment and enzymatic hydrolysis, the maximal total xylose and glucose yields reached 87.1% and 97.96%, respectively, by adding tea saponin (60 mg/g substrate) with reduction of 50% enzyme loading.