The Metabolic Changes between Monolayer (2D) and Three-Dimensional (3D) Culture Conditions in Human Mesenchymal Stem/Stromal Cells Derived from Adipose Tissue.
Cells(2023)
摘要
Our results may suggest that metabolic reconfiguration accompanies the transition from 2D to 3D culture and the processes of both mitochondrial respiration and glycolysis become more active. Intensified metabolism might be associated with the increased demand for energy, which is needed to maintain the expression of pluripotency genes and stemness state.
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关键词
3D culture,ASC,ATP,DFAT,MSC,OXPHOS,glycolysis,hypoxia,metabolism,mitochondria,spheres,spheroid,three-dimensional culture
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